1Shibetsu Salmon Museum, Shibetsu, Hokkaido 086-1631, 2National Research Institute of Far Seas Fisheries, Fisheries Research Agency, Yokohama, Kanagawa, 236-8648, 3National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Kanagawa 236-8648, 4National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, 5Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan
“Sakemasu” collected in the Nemuro Strait was identified as a hybrid between chum salmon Oncorhynchus keta and pink salmon O. gorbuscha using analyses of mtDNA and nuclear DNA. The nucleotide sequences of ATPase6, NADH3 of mtDNA and ITS1, ITS2, and Aromatase B-1 of nuclear DNA of O. keta and O. gorbuscha were compared to construct species specific primers (SSP). The combined results of SSP-PCR analysis of mtDNA and nuclear DNA indicated that six individuals from eleven “Sakemasu” are hybrids between chum and pink salmon. Some morphological features of crossbred “sakemasu” were mixed with parental traits.