Nippon Suisan Gakkaishi 76 (4), 637-645 (2010)

Multiple marking of alizarin complexone for the otolith of nigorobuna Carassius auratus grandoculis and estimation of individual size-at-release

KOH-ICHI FUJIWARA,1,2* TAKAHIRO USUKI,1a
MORIHITO NEMOTO1 AND SHUICHI KITADA2

1Shiga Prefecture Fisheries Experiment Station, Hikone, Shiga 522-0057, 2Faculty of Marine Science, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan

The ALC multiple marking technique to evaluate effective stocking method of nigorobuna Carassius auratus grandoculis in Lake Biwa was examined. Fluorescence multiple rings corresponding to the marking times were clearly identified in the lapillus marked in the eyed-stage of eggs and at intervals of four days or more for five-day-old or older fish, and more various marks were possible by combination with multiple rings. The five-rings mark did not affect growth and survival of the released fish. From the measurement of the outermost ALC luminescence (marked just prior to release) in the otolith of recaptured fish, the standard length of released fish could be back-calculated with sufficient accuracy from the linear-regression equations between long axis length of lapillus (OL; mm) and standard length (SL; mm) of each released group or the formula SL=33.9 OL+521 OL/D+21.2/D−2.69(D: number of days from hatching until release).


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