1Institute of Aquaculture, Kinki University, Shirahama, Wakayama 649- 2211, 2Institute of Aquaculture, Kinki University, Uragami, Wakayama 649- 5145, Japan
The efficacy of immersion-vaccination against gliding bacterial disease in red sea bream Pagrus major was examined with formalin killed cells (FKC) of Tenacibaculum maritimum. FKC was prepared from T. maritimum type strain R2 grown on modified Cytophaga agar medium containing 70% sea water for 24 h at 25°C by inactivating with 1.5% (v/v) formalin-PBS for 24 h at 4°C. In experiment I, juvenile red sea bream immersion-vaccinated with FKC (20 μg/mL) for 30 min were challenged with T. maritimum R2 3 days, 1 and 2 weeks after vaccination. The survival rates of the groups challenged after 1 or 2 weeks were significantly higher than those of the control group (P< 0.05). In experiment II, a twice-vaccinated group was compared with once-vaccinated groups and control. The survival rates of all the vaccinated groups were significantly higher than that of the control (P< 0.05), and the twice-vaccinated group showed the highest survival rate.