Nippon Suisan Gakkaishi 71 (3), 294-298 (2005)

Disinfection of parthenogenetic eggs of the rotifer Brachionus plicatilis

KEN-ICHI WATANABE,1a* DAISUKE SHINOZAKI,2 MASAHIKO KOISO,1
HIROSHI KUWADA1 AND MAMORU YOSHIMIZU2

1Notojima Station, National Center for Stock Enhancement, Fisheries Research Agency, Notojima, Ishikawa 926-0216, 2Graduate School of Fisheries Science, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan

The Disinfectant effect of chemical and physical treatments on parthenogenetic eggs of the rotifer was investigated. No disinfectant effect was observed by treatment with ozonated or electrolyzed seawater, and triton-X under several conditions. Treatments with povidone-iodine at 50 mg/L as iodine, pH 2, and 10,000 mg/L of hydrogen peroxide were effective for disinfection, but hatching rate was low. Glutaraldehyde treatment at the concentration of 1,250 mg/L for 30 min effectively disinfected the eggs of rotifer and did not reduce the hatching rate of parthenogenetic eggs.


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