Nippon Suisan Gakkaishi 70 (1), 60-65 (2004)

Observations of bacteria collected at hygienically controlled Shibetsu fishing port, Japan

HISAE KASAI, EMI SUGIYAMA AND MAMORU YOSHIMIZU

Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan

To control hygienic conditions at fishing ports, qualitative and quantitative bacterial observations have to be considered. In the fishing port located at Shibetsu town, Hokkaido, where the hazard analysis and critical control point (HACCP) system is performed, the distribution and composition of heterotrophic bacterial flora and coliform bacteria were studied in this hygienically controlled site. There was a little difference in the quality of water collected from inside and outside of the harbor. The number of viable bacteria in the surface seawater varied seasonally from 102 to 104 CFU/mL. The generic composition of bacteria collected from back part of the harbor, entrance of the harbor, and the front of the fish market was compared. The generic composition of the bacterial flora included the genera Pseudomonas, Alteromonas and Vibrio, which were observed through the year. Moraxella and gram-positive bacteria were also isolated in front of the fish market. Coliform were isolated inside and outside of the harbor throughout the year, and their counts increased as water temperature increased.


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